Results

• NO production induced by AYPGKF-NH2, thrombin and ionomycin in BAECs

cf. Fig1

①AYPGKF-NH2
Max　30μM
②thrombin
Max 0.1U/ml
③ionomycin
Max 1μM

①=②、③＞①、②
③：ionomycin＞1μM→NO production reduce

• Effects of AYPGKF-NH2,thrombin and ionomycin on [Ca2+]i concentrations in BAECs

cf.Fig2

• • ionomycin; induce a concentration-dependent increase in [Ca2+]i in BAECs
  • AYPGKF-NH2, thrombin;induced no appreciable elevation of [Ca2+]i

• Effects of BAPTA loading on the NO production in BAECs

cf.Fig.3

• Effect on pertussis toxin on the NO production in BAECs
  • AYPGKF-NH2:NO生成を抑制

cf.Fig.4